Magnetic alginate microparticles for purification of .alpha.-amylases.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F03%3A22033023" target="_blank" >RIV/67179843:_____/03:22033023 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Magnetic alginate microparticles for purification of .alpha.-amylases.

Original language description

Spherical magnetic alginate microparticles (25 - 60 .micro.m in diameter) were prepared using the microemulsion system, with water saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solutions. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of .alpha.-amylases. Enzyme activity was eluted by 1.0 M maltose. Alpha-amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96 % activity recovery, respectively.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biotechnology

ISSN

0168-1656

e-ISSN

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Volume of the periodical

105

Issue of the periodical within the volume

N/A

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

255-260

UT code for WoS article

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EID of the result in the Scopus database

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