Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F05%3A00029260" target="_blank" >RIV/67179843:_____/05:00029260 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12640/05:00006502
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803
Original language description
The PsbH protein of cyanobacterium Synechocystis sp. PCC 6803 was expressed as a fusion protein with glutathione-S transferase (GST) in E. coli grown on a mineral medium enriched in 15 N isotope. After enzymatic cleavage of the fusion protein, the H-1-N-15-HSQC spectrum of PsbH protein in presence of the detergent P-D-octyl-glucopyranoside (OG) was recorded on a Bruker DRX 500 MHz NMR spectrometer equipped with a 5 mm TXI cryoprobe to enhance the sensitivity and resolution. Non-labelled protein was usedfor secondary structure estimation by de-convolution from circular dichroism (CD) spectra. Experimental results were compared with our results from a structural model of PsbH using a restraint-based comparative modelling approach combined with moleculardynamics and energetic modelling. We found that PsbH shows 34-38% alpha-helical structure (ThrM-Ser60), a maximum of around 15 % of beta-sheet,and 12-19 % of P-turn.
Czech name
Určení sekundární struktury rekombinantního psbH, fotosyntetického membránového proteinu ze sinice Synechocystis sp. PCC 6803
Czech description
Protein PsbH byl exprimován jako fúzní protein s GST kotvou v normálním médiu a v médiu obohaceném 15N. Byla provedena měření CD a NMR v roztocích s detergentem OG. Byla stanovena struktura 15% ? skládaný list, 38% helix, zbytek ? obrat. Bylo proveden výpočet struktury mimomembránových součástí proteinu, který, při započtení vnitromembránového helixu, dal hodnoty strukrturních motivů ve shodě s experimentem.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
—
Result continuities
Project
—
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Photosynthetica
ISSN
0300-3604
e-ISSN
—
Volume of the periodical
43
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
4
Pages from-to
421-424
UT code for WoS article
—
EID of the result in the Scopus database
—