Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F07%3A00098096" target="_blank" >RIV/67179843:_____/07:00098096 - isvavai.cz</a>

Alternative codes found

RIV/67179843:_____/07:00097985 RIV/68378050:_____/07:00097985 RIV/60076658:12640/07:00008321

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide

Original language description

The flavoprotein WrbA from Escherichia coli is considered to be the prototype of a new family of multimeric flavodoxin-like proteins that are implicated in cell protection against oxidative stress. The present study is aimed at structural characterization of the E. coli protein with respect to its recently revealed oxidoreductase activity. Crystals of WrbA holoprotein in complex with the oxidized flavin cofactor (FMN) were obtained using standard vapour-diffusion techniques. Deep yellow tetragonal crystals obtained from differing crystallization conditions display different space groups and unit-cell parameters. X-ray crystal structures of the WrbA holoprotein have been determined to resolutions of 2.0 and 2.6 angstrom

Czech name

Krystalizace a difrakční analýza E. coli WrbA s navázaným kofaktorem flavinmononukleotidem

Czech description

Flavoprotein WrbA z Escherichia coli je považován za protopyp nové rodiny multimerních proteinů podoných flavodoxinům, které jsou zahrnuty v buněčné ochraně proti oxidativnímu stresu. Předkládaná studie je zaměřena na strukturní charakterizaci WrbA proteinu s ohledem na jeho nalezenou oxidoredukční aktivitu. Krystaly holoproteinu WrbA v komplexu s oxidovaným flavinovým kofaktorem FMN byly získány pomocí standardních krystalizačních technik. Žluté tetragonální krystaly získány z různých podmínek mají odlišnou prostorovou grupu a mřížkové parametry. Difrakční data z krystalů holoproteinu WrbA byly naměřeny do rozlišení 2.0 ? and 2.6 ?.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Crystallographica Section F

ISSN

1744-3091

e-ISSN

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Volume of the periodical

63

Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

5

Pages from-to

571-575

UT code for WoS article

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EID of the result in the Scopus database

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