CN-PAGE as a tool for separating pigment?protein complexes and studying their thermal stability in spruce and barley thylakoid membranes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F15%3A00456344" target="_blank" >RIV/67179843:_____/15:00456344 - isvavai.cz</a>
Alternative codes found
RIV/61988987:17310/15:A1701EXN
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
CN-PAGE as a tool for separating pigment?protein complexes and studying their thermal stability in spruce and barley thylakoid membranes
Original language description
The central aim of our study was to develop a method for solubilization and native electrophoretic (colourless native polyacrylamide gel electrophoresis; CN-PAGE) separation of pigment?protein complexes (PPCs) embedded in thylakoid membranes (tBMs) isolated from spruce. Subsequently, we focused on studying the effect of temperature on the composition and PPC stability of two different species: barley and spruce. We found that the mild detergent n-dodecyl ?-D-maltoside (?-DM) is suitable for PPC solubilization of spruce tBMs, but longer solubilization and a higher ratio of detergent to total chlorophyll are needed for spruce than are needed for barley. We also unified CN-PAGE protocols to optimize the separation of spruce and barley PPCs that resulted in the separation of photosystem I (PSI) and photosystem II (PSII) supercomplexes (SCs), PSI and PSII core dimers, PSII core monomers, trimeric and monomeric light-harvesting complexes of PSII, and bands with free pigments. Studying the ef
Czech name
—
Czech description
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Global Change: A Complex Challenge : Conference Proceedings
ISBN
978-80-87902-10-3
ISSN
—
e-ISSN
—
Number of pages
4
Pages from-to
146-149
Publisher name
Global Change Research Centre, The Czech Academy of Sciences, v. v. i.
Place of publication
Brno
Event location
Brno
Event date
Mar 23, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
—

Similar results(10)