Segmentation Method of Time-Lapse Microscopy Images with the Focus on Biocompatibility Assessment
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985556%3A_____%2F16%3A00460642" target="_blank" >RIV/67985556:_____/16:00460642 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12520/16:43890555 RIV/00216208:11320/16:10324380
Result on the web
<a href="http://dx.doi.org/10.1017/S143192761600074X" target="_blank" >http://dx.doi.org/10.1017/S143192761600074X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S143192761600074X" target="_blank" >10.1017/S143192761600074X</a>
Alternative languages
Result language
angličtina
Original language name
Segmentation Method of Time-Lapse Microscopy Images with the Focus on Biocompatibility Assessment
Original language description
Biocompatibility testing of new materials is often performed in vitro by measuring the growth rate of mammalian cancer cells in time-lapse images acquired by phase contrast microscopes. The growth rate is measured by tracking cell coverage, which requires an accurate automatic segmentation method. However, cancer cells have irregular shapes that change over time, the mottled background pattern is partially visible through the cells and the images contain artifacts such as halos. We developed a novel algorithm for cell segmentation that copes with the mentioned challenges. It is based on temporal differences of consecutive images and a combination of thresholding, blurring and morphological operations. We tested the algorithm on images of four cell types acquired by two different microscopes, evaluated the precision of segmentation against manual segmentation performed by a human operator and finally provided comparison with other freely available methods.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
JD - Use of computers, robotics and its application
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microscopy and Microanalysis
ISSN
1431-9276
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
497-506
UT code for WoS article
000384332600003
EID of the result in the Scopus database
2-s2.0-84964751084