Increased Tissue and Circulating Levels of Dipeptidyl Peptidase-IV Enzymatic Activity in Patients with Pancreatic Ductal Adenocarcinoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985807%3A_____%2F16%3A00460195" target="_blank" >RIV/67985807:_____/16:00460195 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/16:10328039 RIV/61383082:_____/16:00000140 RIV/00064165:_____/16:10328039

Result on the web

<a href="http://dx.doi.org/10.1016/j.pan.2016.06.001" target="_blank" >http://dx.doi.org/10.1016/j.pan.2016.06.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.pan.2016.06.001" target="_blank" >10.1016/j.pan.2016.06.001</a>

Result language

angličtina

Original language name

Increased Tissue and Circulating Levels of Dipeptidyl Peptidase-IV Enzymatic Activity in Patients with Pancreatic Ductal Adenocarcinoma

Original language description

Background/objectives: Pancreatic ductal adenocarcinoma (PDAC) is frequently heralded by an impairment of glucose homeostasis. Dipeptidyl peptidase-IV (DPP-IV) and fibroblast activation protein alpha (FAP) are aminopeptidases that regulate several bioactive peptides involved in glucoregulation, and are frequently dysregulated in cancer. The present study analyzes blood plasma levels and the quantity and localization of DPP-IV and FAP in PDAC tissues. Methods: DPP-IV and FAP concentration and enzymatic activity were evaluated in the plasma from 93 PDAC, 39 type 2 diabetes mellitus (T2DM) and 29 control subjects, and in matched paired non-tumorous and tumor tissues from 48 PDAC patients. The localization of DPP-IV and FAP was determined using immunohistochemistry and catalytic histochemistry. Results: The enzymatic activity and concentration of DPP-IV was higher in PDAC tumor tissues compared to non-tumorous pancreas. DPP-IV was expressed in cancer cells and in the fibrotic stroma by activated (myo)fibroblasts including DPP-IV+FAP+ cells. FAP was expressed in stromal cells and in some cancer cells and its expression was increased in the tumors. Plasmatic DPP-IV enzymatic activity, and in particular the ratio between DPP-IV enzymatic activity and concentration in PDAC with recent onset DM was higher compared to T2DM. In contrast, the plasmatic FAP enzymatic activity was lower in PDAC compared to T2DM and controls and rose after tumor removal. Conclusions: DPP-IV-like enzymatic activity is upregulated in PDAC tissues. PDAC patients with recent onset diabetes or prediabetes have increased plasmatic DPP-IV enzymatic activity. These changes may contribute to the frequently observed association of PDAC and recent onset impairment of glucoregulation.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

—

Project

<a href="/en/project/NT14254" target="_blank" >NT14254: Early Diagnosis of Pancreatic Adenocarcinoma: Surface Proteases and Specific Biomarkers</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Pancreatology

ISSN

1424-3903

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

5

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

829-838

UT code for WoS article

000384785700025

EID of the result in the Scopus database

2-s2.0-84991726484