Improvement in volume estimation from confocal sections after image deconvolution
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F04%3A00103084" target="_blank" >RIV/67985823:_____/04:00103084 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Improvement in volume estimation from confocal sections after image deconvolution
Original language description
The confocal fluorescence microscope is considered as a true volume imager but intrinsic imaging properties of the optical scheme yield a corrupted image that can hamper quantitative analysis of successive image planes. By a post-image collection restoration we obtained an estimate of the true object, utilizing the Point Spread Function (PSF).The PSF was measured and predicted to have a mathematical and physical model of the image-formation process. We used the regularized Iterative Constrained TykhonovMiller restoration algorithm for solving the inverse problem. In order to compare the effective improvement in the quantitative image information analysis, we measured the volume of reference objects before and after image restoration, using the isotropic fakir stereological method
Czech name
Zpřesnění odhadu objemu z konfokálních řezů po aplikaci dekonvoluce obrazu
Czech description
Použili jsme dekonvoluci obrazu pro zpřesnění odhadu objemu z konfokálních řezů. Zpřesnění bylo vyhodnoceno porovnáním odhadů objemů referenčních objektů před a po dekonvoluci s využitím fakírské stereologické metody
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microscopy Research Technique
ISSN
1059-910X
e-ISSN
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Volume of the periodical
64
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
151-155
UT code for WoS article
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EID of the result in the Scopus database
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