All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Improvement in volume estimation from confocal sections after image deconvolution

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F04%3A00103084" target="_blank" >RIV/67985823:_____/04:00103084 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Improvement in volume estimation from confocal sections after image deconvolution

  • Original language description

    The confocal fluorescence microscope is considered as a true volume imager but intrinsic imaging properties of the optical scheme yield a corrupted image that can hamper quantitative analysis of successive image planes. By a post-image collection restoration we obtained an estimate of the true object, utilizing the Point Spread Function (PSF).The PSF was measured and predicted to have a mathematical and physical model of the image-formation process. We used the regularized Iterative Constrained TykhonovMiller restoration algorithm for solving the inverse problem. In order to compare the effective improvement in the quantitative image information analysis, we measured the volume of reference objects before and after image restoration, using the isotropic fakir stereological method

  • Czech name

    Zpřesnění odhadu objemu z konfokálních řezů po aplikaci dekonvoluce obrazu

  • Czech description

    Použili jsme dekonvoluci obrazu pro zpřesnění odhadu objemu z konfokálních řezů. Zpřesnění bylo vyhodnoceno porovnáním odhadů objemů referenčních objektů před a po dekonvoluci s využitím fakírské stereologické metody

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EA - Morphology and cytology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Microscopy Research Technique

  • ISSN

    1059-910X

  • e-ISSN

  • Volume of the periodical

    64

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    5

  • Pages from-to

    151-155

  • UT code for WoS article

  • EID of the result in the Scopus database