Expression of the Saccharomyces cerevisiae MPR1 gene encoding N-acetyltransferase in Zygosaccharomyces rouxii confers resistance to l-azetidine-2-carboxylate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F06%3A00040468" target="_blank" >RIV/67985823:_____/06:00040468 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Expression of the Saccharomyces cerevisiae MPR1 gene encoding N-acetyltransferase in Zygosaccharomyces rouxii confers resistance to l-azetidine-2-carboxylate

Original language description

L-azetidine-2-carboxylate (AZC) is a toxic compound that is detoxified by Saccharomyces cerevisiae Mpr1 and Mpr2 acetyltransferases. The sensitivity of Z. rouxii to AZC was proven, and the use of ScMPR1 as a dominant selection marker in Z. rouxii tested.Although the ScMPR1 expressing cells were AZC-resistant, the detoxification activity was not sufficient to confer resistance on AZC-containing medium immediately after transformation. The same was found for an AZC-sensitive S. cerevisiae strain. In bothyeasts, ScMPR1 can be used only as an auxiliary marker gene

Czech name

Exprese genu MPR1 ze Saccharomyces cerevisiae kódujícího N-acetyltransferasu udělí Zygosaccharomyces rouxii resistenci k L-azetidine-2-carboxylátu

Czech description

Zkoumali jsme možnost využití genu MPR1 ze Saccharomyces cerevisiae jako dominantní selekční značky pro selekci transformantů Zygosaccharomyces rouxii resistentních na normálně toxickou sloučeninu L-azetidine-2-carboxylát. Ukázalo se, že tento gen udělíZ. rouxii resistenci k L-azetidine-2-carboxylátu, nicméně jeho využití jako dominantní selekční značky je znemožněno vysokou frekvencí spontánně rezistentních mutantů. Totéž bylo zjištěno pro S. cerevisiae

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

203-207

UT code for WoS article

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EID of the result in the Scopus database

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