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Identification of collagen types in tissues using HPLC-MS/MS

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F08%3A00315977" target="_blank" >RIV/67985823:_____/08:00315977 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Identification of collagen types in tissues using HPLC-MS/MS

  • Original language description

    A method for the determination and quantification of collagen types I?V in rat tissues has been developed. This method is based on collagen fragmentation by cyanogen bromide followed by trypsin digestion. After that, HPLC-MS/MS analyses of the resultingpeptide mixtures (peptide maps) were performed.The applicability of this method for analyzing collagen types was demonstrated on rat tissues (skin, tendon, and aorta

  • Czech name

    Identifikace kolagenních typů v tkáních pomocí HPLC-MS/MS

  • Czech description

    Byla vyvinuta metoda pro určení a kvantifikaci kolagenních typů I-V v tkáních potkana. Metoda je založena na fragmentaci kolagenu bromkyanem a štěpení trypsinem. Poté je výsledná peptidová směs analyzována pomocí HPLC-MS/MS. Aplikovatelnost metody byla prokázána při analýze kolagenních typů v tkáních potkana (kůže, šlacha, aorta)

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Separation Science

  • ISSN

    1615-9306

  • e-ISSN

  • Volume of the periodical

    31

  • Issue of the periodical within the volume

    20

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    6

  • Pages from-to

  • UT code for WoS article

    261237500001

  • EID of the result in the Scopus database