A System for Automated Acquisition of 3D Biomedical Images Using Confocal Microscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F09%3A00330404" target="_blank" >RIV/67985823:_____/09:00330404 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/09:00157366
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
A System for Automated Acquisition of 3D Biomedical Images Using Confocal Microscopy
Original language description
The described software system for the coarse-to-fine positioning of XY motorized microscope stage allows us interactive control of image acquisition in confocal microscopy. An image captured through a low-resolution objective serves as navigation map where the user points to positions or areas of interest in which a high-resolution image will be captured. Our contribution presents an extension of a two-level coarse/fine algorithm to a multi-level environment. Moreover, the system is accompanied by algorithms that compensate both uneven illumination of neighbouring captured images (neighbouring fields of view) and light attenuation of confocal 3D images with depth, which are effects observed in confocal microscopy. By applying these algorithms resultingimages of high quality are obtained. The system also enables us acquisition of confocal data sets for performing 3D reconstructions of large biological specimens
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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