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Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F09%3A00337821" target="_blank" >RIV/67985823:_____/09:00337821 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata

  • Original language description

    The ENA1 gene encoding plasma membrane Na+ATPase in the pathogenic C. glabrata was amplified by PCR and cloned behind the constitutive Saccharomyces cerevisiae NHA1 promotor into S. cerevisiae/E.coli (URA3/AmpR) shuttle vectors YEp352 or pGRU1 (for expression of Ena1 proteins tagged with GFP at their C termini). Resulting plasmids allow the heterologous expression of Candida glabrata Ena1 transporter in S. cerevisiae strains and the study of its substrate specificity, activity and/or localization

  • Czech name

  • Czech description

Classification

  • Type

    G<sub>funk</sub> - Functional sample

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/LC531" target="_blank" >LC531: Centre on molecular biology and physiology of yeast communities</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Internal product ID

    CandidaENA1-YEP352/pGRU1

  • Numerical identification

  • Technical parameters

    Sterilní vodné roztoky dvou plasmidových DNA určených pro transformaci E.coli (selekce AmpR) nebo S. cerevisiae (selekce Ura+) a počítačové soubory znázorňující sekvence DNA plasmidů

  • Economical parameters

    náklady 300 000,- Kč, licenční poplatek 50 000,- Kč

  • Application category by cost

  • Owner IČO

    67985823

  • Owner name

    Fyziologický ústav AVČR, v.v

  • Owner country

    CZ - CZECH REPUBLIC

  • Usage type

    P - Využití výsledku jiným subjektem je v některých případech možné bez nabytí licence

  • Licence fee requirement

    N - Poskytovatel licence na výsledek nepožaduje licenční poplatek

  • Web page