Distribution of mitochondrial DNA nucleoids inside the linear tubules vs. bulk parts of mitochondrial network as visualized by 4Pi microscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F15%3A00446711" target="_blank" >RIV/67985823:_____/15:00446711 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10863-015-9610-3" target="_blank" >http://dx.doi.org/10.1007/s10863-015-9610-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10863-015-9610-3" target="_blank" >10.1007/s10863-015-9610-3</a>

Result language
angličtina
Original language name
Distribution of mitochondrial DNA nucleoids inside the linear tubules vs. bulk parts of mitochondrial network as visualized by 4Pi microscopy
Original language description
To visualize nucleoid distribution within the mt reticular network, we have employed three?dimensional (3D) double?color 4Pi microscopy. The mt network was visualized in hepatocellular carcinoma HepG2 cells via mt?matrix?addressed GFP, while 3D immunocytochemistry of mtSSB was performed. Optimization of iso-surface computation threshold for nucleoid 4Pi images to 30 led to an average nucleoid diameter of 219110 nm and 224100 nm in glucose? and galactose?cultivated HepG2 cells (the latter with obligatoryoxidative phosphorylation). We have positioned mtDNA nucleoids within the mt reticulum network and refined our model for nucleoid redistribution within the fragmented network ?? clustering of up to 10 nucleoids in 2 m diameter mitochondrial spheroids ofa fragmented mt network, arising from an original 10 m mt tubule of a 400 nm diameter. However, the theoretically fragmented bulk parts were observed most frequently as being reintegrated into the continuous mt network in 4Pi images. Sin
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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