Coupled aggregation of mitochondrial single-strand DNA-binding protein tagged with Eos fluorescent protein visualizes synchronized activity of mitochondrial nucleoids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F15%3A00449774" target="_blank" >RIV/67985823:_____/15:00449774 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3892/mmr.2015.4085" target="_blank" >http://dx.doi.org/10.3892/mmr.2015.4085</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/mmr.2015.4085" target="_blank" >10.3892/mmr.2015.4085</a>

Result language

angličtina

Original language name

Coupled aggregation of mitochondrial single-strand DNA-binding protein tagged with Eos fluorescent protein visualizes synchronized activity of mitochondrial nucleoids

Original language description

A small limited number of large aggregates of mtSSB EosFP tagged protein started to form in the cells, which exhibited a great co localization with the noted coupled positions. Antibody staining and 3D immunocytochemistry confirmed that nucleoid components such as TFAM and DNA were co localized with these aggregates. Furthermore, the observed reduction of the mtDNA copy number in mtSSB EosFP transfected cells suggested a possible impairment of nucleoid function. In conclusion, the present study demonstrated that coupled nucleoids are synchronized by mtSSB EosFP overexpression and visualized through their equal binding capacity to mtSSB EosFP tagged protein. This observation suggested parallel replication and transcription activity of nucleoid couples native from a parental one. Preserved coupling in late stages of artificial EosFP mediated aggregation of tagged proteins suggested a rational manner of mitochondrial branching that may be cell-type specifically dependent on hierarchical n

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Medicine Reports

ISSN

1791-2997

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

4

Country of publishing house

GR - GREECE

Number of pages

6

Pages from-to

5185-5190

UT code for WoS article

000362999200051

EID of the result in the Scopus database

2-s2.0-84940564235