Influence of early life status epilepticus on the developmental expression profile of the GluA2 subunit of AMPA receptors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00465392" target="_blank" >RIV/67985823:_____/16:00465392 - isvavai.cz</a>

Alternative codes found

RIV/00023752:_____/16:43915188 RIV/00216208:11130/16:10327801

Result on the web

<a href="http://dx.doi.org/10.1016/j.expneurol.2016.05.039" target="_blank" >http://dx.doi.org/10.1016/j.expneurol.2016.05.039</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.expneurol.2016.05.039" target="_blank" >10.1016/j.expneurol.2016.05.039</a>

Result language

angličtina

Original language name

Influence of early life status epilepticus on the developmental expression profile of the GluA2 subunit of AMPA receptors

Original language description

At early stages of postnatal development, the majority of AMPARs are permeable to both Na(+) and Ca(2+) ions. This permeability, which increases neuronal excitability, is due to the lack of the GluA2 subunit, encoded by the GRIA2A gene, and/or the presence of an unedited GluA2 subunit Q/R site (glutamine instead of arginine). Lithium chloride- and pilocarpine-induced status epilepticus (LiCl/Pilo-SE) in rodents represents a model of severe seizures that result in development of temporal lobe epilepsy (TLE). The aim of this study was to determine how LiCl/Pilo-SE induced early in life (at postnatal day 12; P12) alters normal expression of the GRIA2A gene and GluA2 protein. There was a significant increase in GRIA2A mRNA expression in the CXFR, CXPAR, and CXOC of P18 SE animals. In CXFR and HD, increased expression of GluA2 AMPAR subunit protein was detected, as well as a surge in GRIA2A mRNA and GluA2 protein expression especially at P18. In HD the surge was detected not only during development (P18), but also later in life (P72). A marked decrease in the overall expression of GluA2 protein in the HV in the LiCl/Pilo-SE and LiCl/Para rats, suggests that the HV is predisposed to excitotoxicity, not only during development, but even in adulthood. Interestingly, LiCl in combination with paraldehyde can also strongly alter the normal ontogeny of GRIA2A mRNA as well as GluA2 subunit protein expression.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Experimental Neurology

ISSN

0014-4886

e-ISSN

—

Volume of the periodical

283

Issue of the periodical within the volume

Part A

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

97-109

UT code for WoS article

000383314500009

EID of the result in the Scopus database

2-s2.0-84973882800