Characterization of the Candida albicans Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an S-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00469461" target="_blank" >RIV/67985823:_____/16:00469461 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1128/mSphere.00284-16" target="_blank" >http://dx.doi.org/10.1128/mSphere.00284-16</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/mSphere.00284-16" target="_blank" >10.1128/mSphere.00284-16</a>

Result language

angličtina

Original language name

Characterization of the Candida albicans Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an S-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis

Original language description

Amino acids are key sources of nitrogen for growth of Candida albicans. In order to detect and take up these amino acids from a broad range of different and changing nitrogen sources inside the host, this fungus must be able to adapt via its expression of genes for amino acid uptake and further metabolism. We analyzed six C. albicans putative general amino acid permeases based on their homology to the Saccharomyces cerevisiae Gap1 general amino acid permease. We generated single- and multiple-deletion strains and found that, based on growth assays and transcriptional or posttranscriptional regulation, Gap2 is the functional orthologue to ScGap1, with broad substrate specificity. Expression analysis showed that expression of all GAP genes is under control of the Csy1 amino acid sensor, which is different from the situation in S. cerevisiae. We show that Gap4 is the functional orthologue of ScSam3, the only S-adenosylmethionine (SAM) transporter in S. cerevisiae, and we report that Gap4 is required for SAM-induced morphogenesis.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

—

Project

<a href="/en/project/GA16-03398S" target="_blank" >GA16-03398S: Transporters of the pathogenic Candida yeast species as a target for the development of new antifungal drugs.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

mSphere

ISSN

2379-5042

e-ISSN

—

Volume of the periodical

1

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

19

Pages from-to

—

UT code for WoS article

000392587800012

EID of the result in the Scopus database

—