Induction of lipogenesis in white fat during cold exposure in mice: link to lean phenotype

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00474022" target="_blank" >RIV/67985823:_____/17:00474022 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/ijo.2016.228" target="_blank" >http://dx.doi.org/10.1038/ijo.2016.228</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/ijo.2016.228" target="_blank" >10.1038/ijo.2016.228</a>

Result language

angličtina

Original language name

Induction of lipogenesis in white fat during cold exposure in mice: link to lean phenotype

Original language description

Futile substrate cycling based on lipolytic release of fatty acids (FA) from intracellular triacylglycerols (TAG) and their re-esterification (TAG/FA cycling), as well as de novo FA synthesis (de novo lipogenesis (DNL)), represent the core energy-consuming biochemical activities of white adipose tissue (WAT). We aimed to characterize their roles in cold-induced thermogenesis and energy homeostasis.Male obesity-resistant A/J and obesity-prone C57BL/6J mice maintained at 30 degrees C were exposed to 6 degrees C for 2 or 7 days. In epididymal WAT (eWAT), TAG synthesis and DNL were determined using in vivo H-2 incorporation from (H2O)-H-2 into tissue TAG and nuclear magnetic resonance spectroscopy. Quantitative real-time-PCR and/or immunohistochemistry and western blotting were used to determine the expression of selected genes and proteins in WAT and liver.The mass of WAT depots declined during cold exposure (CE). Plasma levels of TAG and non-esterified FA were decreased by day 2 but tended to normalize by day 7 of CE. TAG synthesis (reflecting TAG/FA cycle activity) gradually increased during CE. DNL decreased by day 2 of CE but increased several fold over the control values by day 7. Expression of genes involved in lipolysis, glyceroneogenesis, FA re-esterification, FA oxidation and mitochondrial biogenesis in eWAT was induced during CE. All these changes were more pronounced in obesity-resistant A/J than in B6 mice and occurred in the absence of uncoupling protein 1 in eWAT. Leptin and fibroblast growth factor 21 plasma levels were differentially affected by CE in the two mouse strains. Our results indicate integrated involvement of (i) TAG/FA cycling and DNL in WAT, and (ii) hepatic very-low-density lipoprotein-TAG synthesis in the control of blood lipid levels and provision of FA fuels for thermogenesis in cold. They suggest that lipogenesis in WAT contributes to a lean phenotype.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Obesity

ISSN

0307-0565

e-ISSN

—

Volume of the periodical

41

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

372-380

UT code for WoS article

000395816000003

EID of the result in the Scopus database

2-s2.0-85009827360