Lipophilic phosphorescent gold(I) clusters as selective probes for visualization of lipid droplets by two-photon microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00481073" target="_blank" >RIV/67985823:_____/17:00481073 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.jphotochem.2016.08.017" target="_blank" >http://dx.doi.org/10.1016/j.jphotochem.2016.08.017</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jphotochem.2016.08.017" target="_blank" >10.1016/j.jphotochem.2016.08.017</a>

Result language

angličtina

Original language name

Lipophilic phosphorescent gold(I) clusters as selective probes for visualization of lipid droplets by two-photon microscopy

Original language description

The development of organelle-specific phosphorescent multiphoton probes is a rapidly growing area in biomedical imaging. Phosphorescent properties allow spectral or time-resolved discrimination from autofluorescence, while the use of two-photon microscopy provides higher spatial resolution as well as deeper penetration into the sample compared to conventional microscopies. The main challenge in this area is the design of probes that combine a complex of desired properties. Here we present investigation of the phosphorescent homoleptic alkynyl gold(I) cluster, (AuC2R)(10) (R 2,6-dimethyl-4-heptanol), which combines nonlinear optical properties with selectivity to lipid droplets of adipocytes and other cells. This label reveals high two-photon absorption and emission cross-sections in hexane, good solubility in triglycerides (vegetable oil) and microsecond lifetimes both in solution and in biological tissues. These features make it a promising probe for multiphoton bioimaging as demonstrated by successful visualization of various adipose tissues (subcutaneous fat, visceral adipose tissue, adipose tissue of mesentery) of different animals (pigeon, chicken, mouse) as well as lipid droplets in Hep G2 cells. The complex demonstrates high selectivity to lipid droplets in animal tissues and cells. The probe lifetimes in microsecond domain made possible its visualization using phosphorescent lifetime imaging (PLIM) techniques.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Project

<a href="/en/project/LM2015062" target="_blank" >LM2015062: National Infrastructure for Biological and Medical Imaging</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Photochemistry and Photobiology A-Chemistry

ISSN

1010-6030

e-ISSN

—

Volume of the periodical

332

Issue of the periodical within the volume

Jan 1

Country of publishing house

CH - SWITZERLAND

Number of pages

9

Pages from-to

122-130

UT code for WoS article

000387837300014

EID of the result in the Scopus database

2-s2.0-84983444485