Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00508556" target="_blank" >RIV/67985823:_____/19:00508556 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/jbm.a.36754" target="_blank" >https://doi.org/10.1002/jbm.a.36754</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jbm.a.36754" target="_blank" >10.1002/jbm.a.36754</a>
Alternative languages
Result language
angličtina
Original language name
Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering
Original language description
The present work focuses on the development of novel multicomponent organic-inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely alpha-TCP in various concentrations (0-70 wt%) was proposed. The results showed that alpha-TCP underwent incomplete transformation to calcium-deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30-70 wt% alpha-TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of alpha-TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14-day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast-like MG-63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Result continuities
Project
<a href="/en/project/GBP108%2F12%2FG108" target="_blank" >GBP108/12/G108: Preparation, modification and characterization of materials by radiation</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biomedical Materials Research. Part A
ISSN
1549-3296
e-ISSN
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Volume of the periodical
107
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
2479-2491
UT code for WoS article
000477468100001
EID of the result in the Scopus database
2-s2.0-85069939211