Gradual cold acclimation induces cardioprotection without affecting adrenergic beta-receptor-mediated adenylyl cyclase signaling
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F20%3A00524244" target="_blank" >RIV/67985823:_____/20:00524244 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10413518
Result on the web
<a href="https://doi.org/10.1152/japplphysiol.00511.2019" target="_blank" >https://doi.org/10.1152/japplphysiol.00511.2019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1152/japplphysiol.00511.2019" target="_blank" >10.1152/japplphysiol.00511.2019</a>
Alternative languages
Result language
angličtina
Original language name
Gradual cold acclimation induces cardioprotection without affecting adrenergic beta-receptor-mediated adenylyl cyclase signaling
Original language description
Novel strategies are needed that can stimulate endogenous signaling pathways to protect the heart from myocardial infarction. The present study tested the hypothesis that appropriate regimen of cold acclimation (CA) may provide a promising approach for improving myocardial resistance to ischemia/reperfusion (UR) injury without negative side effects. We evaluated myocardial UR injury, mitochondrial swelling, and beta-adrenergic receptor (beta-AR)-adenylyl cyclase-mediated signaling. Male Wistar rats were exposed to CA (8 degrees C, 8 h/day for a week, followed by 4 wk at 8 degrees C for 24 h/day), while the recovery group (CAR) was kept at 24 degrees C for an additional 2 wk. The myocardial infarction induced by coronary occlusion for 20 min followed by 3-h reperfusion was reduced from 56% in controls to 30% and 23% after CA and CAR. respectively. In line, the rate of mitochondrial swelling at 200 mu M Ca2+ was decreased in both groups. Acute administration of metoprolol decreased infarction in control group and did not affect the CA-elicited cardiprotection. Accordingly, neither beta 1-AR-G(s)alpha-adenyly-1- cyclase signaling. stimulated with specific ligands, nor p-PKA/PICA ratios were affected after CA or CAR. Importantly. Western blot and immunofluorescence analyses revealed beta 2- and beta 3-AR protein enrichment in membranes in both experimental groups. We conclude that gradual cold acclimation results in a persisting increase of myocardial resistance to I/R injury without hypertension and hypertrophy. The cardioprotective phenotype is associated with unaltered adenylyl cyclase signaling and increased mitochondrial resistance to Ca2+-overload. The potential role of upregulated beta 2/beta 3-AR pathways remains to be elucidated.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
<a href="/en/project/GA17-07748S" target="_blank" >GA17-07748S: Cardioprotective potential of cold acclimation in rats: the role of uncoupling proteins.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Physiology
ISSN
8750-7587
e-ISSN
—
Volume of the periodical
128
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1023-1032
UT code for WoS article
000528322200031
EID of the result in the Scopus database
2-s2.0-85083545249