Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer Cells in Mouse Liver

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F20%3A00534399" target="_blank" >RIV/67985823:_____/20:00534399 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007%2F978-1-0716-0704-6_8" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-0716-0704-6_8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-0704-6_8" target="_blank" >10.1007/978-1-0716-0704-6_8</a>

Result language
angličtina
Original language name
Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer Cells in Mouse Liver
Original language description
Here, we describe a protocol to prepare and administer glucan-encapsulated RNAi particles (GeRPs), for specific delivery of siRNA and subsequent gene silencing in Kupffer cells (KCs) in mice. This technology is based on baker’s yeast and allows gene manipulation in macrophages in a tissue-specific manner depending on the route of administration and the model that is used. GeRP administered by intravenous injection in mice are delivered to KCs. Therefore, using the GeRP technology to silence genes provides a unique method to study the function of factors expressed by KCs in the regulation of liver function.
Czech name
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Czech description
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Project
<a href="/en/project/GA20-03586S" target="_blank" >GA20-03586S: Bone marrow adipose tissue (BMAT) as a novel adipose depot in the regulation of the whole body metabolism and bone homeostasis.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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