Tissue clearing and imaging methods for cardiovascular development
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00542872" target="_blank" >RIV/67985823:_____/21:00542872 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/21:10428907
Result on the web
<a href="https://doi.org/10.1016/j.isci.2021.102387" target="_blank" >https://doi.org/10.1016/j.isci.2021.102387</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.isci.2021.102387" target="_blank" >10.1016/j.isci.2021.102387</a>
Alternative languages
Result language
angličtina
Original language name
Tissue clearing and imaging methods for cardiovascular development
Original language description
Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different tissue clearing methods for 3D imaging of heart and vasculature, based on chemical composition (solvent-based, simple immersion, hyperhydration, and hydrogel embedding techniques). We discuss in detail compatibility of various tissue clearing techniques with visualization methods: fluorescence preservation, immunohistochemistry, nuclear staining, and fluorescent dyes vascular perfusion. We also discuss myocardium visualization using autofluorescence, tissue shrinking, and expansion. Then we overview imaging methods used to study cardiovascular system and live imaging. We discuss heart and vessels segmentation methods and image analysis. The review covers the whole process of cardiovascular system 3D imaging, starting from tissue clearing and its compatibility with various visualization methods to the types of imaging methods and resulting image analysis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30201 - Cardiac and Cardiovascular systems
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
iScience
ISSN
2589-0042
e-ISSN
2589-0042
Volume of the periodical
24
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
25
Pages from-to
102387
UT code for WoS article
000642261700126
EID of the result in the Scopus database
2-s2.0-85104350157