The Lysosome Origin of Biosilica Machinery in the Demospongiae Model Petrosia ficiformis (Poiret, 1789)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00557425" target="_blank" >RIV/67985823:_____/22:00557425 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3389/fmars.2022.850653" target="_blank" >https://doi.org/10.3389/fmars.2022.850653</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmars.2022.850653" target="_blank" >10.3389/fmars.2022.850653</a>
Alternative languages
Result language
angličtina
Original language name
The Lysosome Origin of Biosilica Machinery in the Demospongiae Model Petrosia ficiformis (Poiret, 1789)
Original language description
The silicification mechanism in sponges is a biologically controlled process where the complex and amazing shape of spicules is the result of the hierarchical assembly of silicon particles to form a composite structure with organic compounds, mainly constituted by proteins. In this work, using an integrated approach of transcriptomic and proteomic analysis, we describe the protein content of sponge spicules in the marine demosponge Petrosia ficiformis (Poiret, 1789). Proteins from spicules were obtained via an ammonium fluoride extraction procedure to remove the inorganic silica followed by SDS-PAGE electrophoresis. The resulting data of LC-MS/MS analysis of the extracted SDS-PAGE bands were then processed with the MASCOT software to search against a database consisting of transcripts and predicted proteins of P. ficiformis. The results revealed a very heterogeneous group of 21 proteins, including silicatein beta, different isoforms of cathepsins, proteins with strong homologies with enzymes like sulphatases, glycosidases, lipid-related proteins, phosphatases, and some others with unknown function. Most of the proteins found here have structures and domains attributable to lysosomes enzymes and for this reason it could be related to these cellular structures the evolutionary origin of the biosilica machinery in sponges.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA20-03899S" target="_blank" >GA20-03899S: Affinity capillary electrokinetic methods for selective analysis of biopolymers and metabolites and for study of their interactions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in marine science
ISSN
2296-7745
e-ISSN
2296-7745
Volume of the periodical
9
Issue of the periodical within the volume
Apr 13
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
850653
UT code for WoS article
000791968300001
EID of the result in the Scopus database
2-s2.0-85128866786