Adipose tissue-specific ablation of PGC-1 beta impairs thermogenesis in brown fat

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00557788" target="_blank" >RIV/67985823:_____/22:00557788 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1242/dmm.049223" target="_blank" >https://doi.org/10.1242/dmm.049223</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/dmm.049223" target="_blank" >10.1242/dmm.049223</a>

Result language

angličtina

Original language name

Adipose tissue-specific ablation of PGC-1 beta impairs thermogenesis in brown fat

Original language description

Impaired thermogenesis observed in mice with whole-body ablation of peroxisome proliferator-activated receptor-gamma coactivator-1 beta (PGC-1 beta, officially known as PPARGC1B) may result from impaired brown fat (brown adipose tissue, BAT) function, but other mechanism(s) could be involved. Here, using adipose-specific PGC-1 beta knockout mice (PGC-1 beta-AT-KO mice) we aimed to learn whether specific PGC-1 beta ablation in adipocytes is sufficient to drive cold sensitivity. Indeed, we found that warm-adapted (30 degrees C) mutant mice were relatively sensitive to acute cold exposure (6 degrees C). When these mice were subjected to cold exposure for 7 days (7-day-CE), adrenergic stimulation of their metabolism was impaired, despite similar levels of thermogenic uncoupling protein 1 in BAT in PGC-1 beta-AT-KO and wild-type mice. Gene expression in BAT of mutant mice suggested a compensatory increase in lipid metabolism to counteract the thermogenic defect. Interestingly, a reduced number of contacts between mitochondria and lipid droplets associated with low levels of L-form of optic atrophy 1 was found in BAT of PGC-1 beta-AT-KO mice. These genotypic differences were observed in warm-adapted mutant mice, but they were partially masked by 7-day-CE. Collectively, our results suggest a role for PGC-1 beta in controlling BAT lipid metabolism and thermogenesis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Disease Models & Mechanisms

ISSN

1754-8403

e-ISSN

1754-8411

Volume of the periodical

15

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

dmm049223

UT code for WoS article

000796546700007

EID of the result in the Scopus database

2-s2.0-85128801831