The co-culture of keratinocytes and fibroblasts on a multi-layered polyester nanofibrous membrane enriched with platelet lysate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00567464" target="_blank" >RIV/67985823:_____/22:00567464 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/21:00567464

Result on the web

<a href="https://www.confer.cz/nanocon/2021/read/4364-the-co-culture-of-keratinocytes-and-fibroblasts-on-a-multi-layered-polyester-nanofibrous-membrane-enriched-with-platelet-lysate.pdf" target="_blank" >https://www.confer.cz/nanocon/2021/read/4364-the-co-culture-of-keratinocytes-and-fibroblasts-on-a-multi-layered-polyester-nanofibrous-membrane-enriched-with-platelet-lysate.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2021.4364" target="_blank" >10.37904/nanocon.2021.4364</a>

Result language

angličtina

Original language name

The co-culture of keratinocytes and fibroblasts on a multi-layered polyester nanofibrous membrane enriched with platelet lysate

Original language description

The prevalence of chronic wounds is increasing due to the population ageing and specific illnesses like diabetes mellitus and vascular diseases. Nanofibrous membranes fabricated using synthetic polymers are promising materials to enhance skin wound healing. PCL and PVA membranes are being studied to be used as scaffolds for skin tissue engineering and hydrogels for controlled drug delivery, respectively. The present study considers the development of a multi-layered membrane made of PCL and PVA loaded with platelet lysate (PL). PCL nanofibers allowed cell adhesion and growth, whereas PVA acted as a hydrogel that releases the bioactive compounds of platelet lysate. The cytocompatibility of the membranes containing PL and without it was demonstrated on two cell types involved in wound healing, i.e. keratinocytes and fibroblasts. Both cell types were able to adhere and proliferate on the membranes. In addition, the membrane containing PL enhanced the proliferation of fibroblasts. A co-culture study was also performed by seeding each cell type on one side of the membrane. The cells were co-cultured for 7 days and the results showed that PL increased the proliferation of cells achieving a monolayer of keratinocytes or fibroblasts on each side of the membrane. Thus, the beneficial effect of PCL-PVA+PL membranes on monocultures and co-cultures of skin cells was demonstrated, and these membranes can be considered potential scaffolds for treatment of chronic wounds.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

<a href="/en/project/NV18-01-00332" target="_blank" >NV18-01-00332: Treatment of diabetic wounds using nanofibrous dressings releasing platelet lysate components.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON 2021 - Conference proceedings

ISBN

978-80-88365-00-6

ISSN

2694-930X

e-ISSN

—

Number of pages

6

Pages from-to

234-239

Publisher name

Tanger Ltd.

Place of publication

Ostrava

Event location

Brno

Event date

Oct 20, 2021

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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