The Circadian Clock of Polarized Microglia and Its Interaction with Mouse Brain Oscillators
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F23%3A00570554" target="_blank" >RIV/67985823:_____/23:00570554 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10446147
Result on the web
<a href="https://doi.org/10.1007/s10571-022-01252-1" target="_blank" >https://doi.org/10.1007/s10571-022-01252-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10571-022-01252-1" target="_blank" >10.1007/s10571-022-01252-1</a>
Alternative languages
Result language
angličtina
Original language name
The Circadian Clock of Polarized Microglia and Its Interaction with Mouse Brain Oscillators
Original language description
The activity of the immune system is controlled by circadian clocks present in different immune cells. The brain-resident subtype of immune cells, microglia, exhibits a wide range of functional phenotypes depending on the signaling molecules in their microenvironment. The exact role of microglia in the hypothalamic suprachiasmatic nuclei (SCN), the central circadian clock, has not been known. Therefore, the aim of this study was to determine (1) whether microenvironment-induced changes in microglial polarization affect circadian clocks in these cells and (2) whether the presence of microglia contributes to SCN clock function. Microglial and SCN clocks were monitored using PER2-driven bioluminescence rhythms at the tissue and single-cell levels. We found that polarization of resting microglia to a pro-inflammatory (M1) or anti-inflammatory (M2) state significantly altered the period and amplitude of their molecular circadian clock, importantly, the parameters changed plastically with the repolarization of microglia. This effect was reflected in specific modulations of the expression profiles of individual clock genes in the polarized microglia. Depletion of microglia significantly reduced the amplitude of the SCN clock, and co-cultivation of the SCN explants with M2-polarized microglia specifically improved the amplitude of the SCN clock. These results demonstrate that the presence of M2-polarized microglia has beneficial effects on SCN clock function. Our results provide new insight into the mutual interaction between immune and circadian systems in the brain.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cellular and Molecular Neurobiology
ISSN
0272-4340
e-ISSN
1573-6830
Volume of the periodical
43
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
1319-1333
UT code for WoS article
000823330100001
EID of the result in the Scopus database
2-s2.0-85134043046