NADPH oxidase 4 in mouse β cells participates in inflammation on chronic nutrient overload

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F24%3A00582386" target="_blank" >RIV/67985823:_____/24:00582386 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/24:10472638 RIV/00216208:11310/24:10472638 RIV/61989592:15110/24:73622312

Result on the web

<a href="https://doi.org/10.1002/oby.23956" target="_blank" >https://doi.org/10.1002/oby.23956</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/oby.23956" target="_blank" >10.1002/oby.23956</a>

Result language

angličtina

Original language name

NADPH oxidase 4 in mouse β cells participates in inflammation on chronic nutrient overload

Original language description

Objective:By exposing mice carrying a deletion of NADPH oxidase isoform 4, NOX4, specifically in pancreatic β cells (βNOX4−/−) to nutrient excess stimulated by a high-fat diet (HFD), this study aimed to elucidate the role of β-cell redox status in the development of meta-inflammation within the diabetic phenotype.Methods:The authors performed basic phenotyping of βNOX4−/− mice on HFD involving insulin and glycemic analyses, histochemistry of adipocytes, indirect calorimetry, and cytokine analyses. To characterize local inflammation, the study used caspase-1 activity assay, interleukin-1β immunochemistry, and real-time polymerase chain reaction during coculturing of β cells with macrophages.Results:The phenotype of βNOX4−/− mice on HFD was not associated with hyperinsulinemia and hyperglycemia but showed accumulation of excessive lipids in epididymal fat and β cells. Surprisingly, mice showed significantly reduced systemic inflammation. Decreased interleukin-1β protein levels and downregulated NLRP3-inflammasome activity were observed on chronic glucose overload in βNOX4−/− isolated islets and NOX4-silenced INS1-E cells resulting in attenuated proinflammatory polarization of macrophages/monocytes in vitro and in situ and reduced local islet inflammation.Conclusions:Experimental evidence suggests that NOX4 pro-oxidant activity in β cells is involved in NLRP3-inflammasome activation during chronic nutrient overload and participates in local inflammatory signaling and perhaps toward peripheral tissues, contributing to a diabetic inflammatory phenotype.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Obesity

ISSN

1930-7381

e-ISSN

1930-739X

Volume of the periodical

32

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

339-351

UT code for WoS article

001122685600001

EID of the result in the Scopus database

2-s2.0-85179336490