Analysis of Surface Expression of NMDAR Subunits in Primary Hippocampal Neurons
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F24%3A00602788" target="_blank" >RIV/67985823:_____/24:00602788 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/978-1-0716-3830-9_3" target="_blank" >https://doi.org/10.1007/978-1-0716-3830-9_3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-3830-9_3" target="_blank" >10.1007/978-1-0716-3830-9_3</a>
Alternative languages
Result language
angličtina
Original language name
Analysis of Surface Expression of NMDAR Subunits in Primary Hippocampal Neurons
Original language description
The expression and activity of ionotropic glutamate receptors control signal transduction at the excitatory synapses in the CNS. The NMDAR comprises two obligatory GluN1 subunits and two GluN2 or GluN3 subunits in different combinations. Each GluN subunit consists of four domains: the extracellular amino-terminal and agonist-binding domains, the transmembrane domain, and the intracellular C-terminal domain (CTD). The CTD interaction with various classes of intracellular proteins is critical for trafficking and synaptic localization of NMDARs. Amino acid mutations or the inclusion of premature stop codons in the CTD could contribute to the emergence of neurodevelopmental and neuropsychiatric disorders. Here, we describe the method of preparing primary hippocampal neurons and lentiviral particles expressing GluN subunits that can be used as a model to study cell surface expression and synaptic localization of NMDARs. We also show a simple method of fluorescence immunostaining of eGFP-tagged GluN2 subunits and subsequent microscopy technique and image analysis to study the effects of disease-associated mutations in the CTDs of GluN2A and GluN2B subunits.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
NMDA Receptors: Methods and Protocols
ISBN
978-1-0716-3829-3
Number of pages of the result
18
Pages from-to
29-46
Number of pages of the book
292
Publisher name
Humana
Place of publication
New York
UT code for WoS chapter
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