Status epilepticus‑induced neuronal degeneration in the immature rat zona incerta is confined to its rostral sector

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F25%3A00619559" target="_blank" >RIV/67985823:_____/25:00619559 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/25:10497320 RIV/00216208:11130/25:10497320

Result on the web

<a href="https://doi.org/10.55782/ane-2024-2447" target="_blank" >https://doi.org/10.55782/ane-2024-2447</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.55782/ane-2024-2447" target="_blank" >10.55782/ane-2024-2447</a>

Result language

angličtina

Original language name

Status epilepticus‑induced neuronal degeneration in the immature rat zona incerta is confined to its rostral sector

Original language description

The distribution and morphology of neuronal degeneration were observed and analyzed in each sector of the zona incerta in a lithium-pilocarpine (LiCl) Wistar rat model of status epilepticus in 12, 15, 18, 21, and 25-day-old rats and survival intervals of 4, 8, 12, 24, and 48 hours. Status epilepticus was induced via intraperitoneal (IP) injection of LiCl (3 mmol/kg) 24 hours before an injection of pilocarpine (40 mg/kg, IP). Motor seizures were suppressed by paraldehyde (0.3-0.6 ml/kg, IP) two hours after status epilepticus onset. Animals were anesthetized using urethane and perfused with phosphate-buffered saline followed by 4% paraformaldehyde. Brains were sectioned and Nissl stained for map guidance, with fluoro-Jade B fluorescence used to detect degenerated neurons. Fluoro-jade B-positive neurons were plotted to a standard stereotaxic atlas, their distribution was quantified, and their long-axis diameter was measured. Fluoro-jade B-positive neurons were found in pups aged 15 days and older 24 hours after status epilepticus, in which their numbers increased, and their perikaryon size decreased with advancing age. Thus, neuronal damage severity was dependent on age and survival interval. Neuronal damage was only found in the rostral sector of the zona incerta, a region that exhibits a small number of inhibitory neurons and is reciprocally connected to the limbic cortex. This system of hyperactivity, coupled with inhibitory neurons, may be the underlying cause of the neuronal degeneration and explain why it was confined to the rostral sector of the zona incerta.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Neurobiologiae Experimentalis

ISSN

0065-1400

e-ISSN

1689-0035

Volume of the periodical

85

Issue of the periodical within the volume

1

Country of publishing house

PL - POLAND

Number of pages

11

Pages from-to

38-48

UT code for WoS article

001473390800004

EID of the result in the Scopus database

2-s2.0-105003706697