The vacuum cast embedding and X-ray micro-computed tomography as standard methods to visualise bioerosion traces in Serpulid tubes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985831%3A_____%2F22%3A00558595" target="_blank" >RIV/67985831:_____/22:00558595 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The vacuum cast embedding and X-ray micro-computed tomography as standard methods to visualise bioerosion traces in Serpulid tubes
Original language description
Bioerosion in tubes of serpulid polychaetes from the late Cenomanian and early Turonian of the Bohemian Cretaceous Basin in the Czech Republic and the late Cenomanian of Le Mans region in France were studied by combination of non-invasive micro-computed tomography and invasive vacuum cast-embedding technique producing polymer resin casts. Our study shows how different imaging techniques bias the description and diagnosis of bioerosion inside tiny serpulid tubes (0.5–0.8 mm in diameter). The great benefits of micro-CT are its non-destructive nature and ability to display empty borings (air space) or borings filled with dense matrix. The results, however, depend critically on the contrast in density and atomic number between the fossil material and the respective boring infilling. Moreover, resolutions of less than 5 μm cannot be effectively achieved. Vacuum cast-embedding is the process of filling tunnels with epoxy resin under vacuum conditions, and dissolving the substrate via treatment with diluted hydrochloric acid. This method is thus unsuitable for poorly soluble (e.g. silicified, pyritized) substrates, boreholes filled with non-porous material, and due to its destructive nature for unique or rare specimens. Nevertheless, the SEM images of the resulting casts have significantly better resolution than micro-CT and thus enable to display and quantify the most delicate morphological features such as microborings as well as details of macroborings.n
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10506 - Paleontology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů