Functional analysis of two genes coding for distinct cation diffusion facilitators of the cadmium-accumulating fungus Agaricus crocodilinus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985831%3A_____%2F25%3A00617167" target="_blank" >RIV/67985831:_____/25:00617167 - isvavai.cz</a>
Alternative codes found
RIV/61389005:_____/25:00617718 RIV/60461373:22330/25:43931634 RIV/60461373:22810/25:43931634
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1878614625000169?dgcid=coauthor" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1878614625000169?dgcid=coauthor</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.funbio.2025.101550" target="_blank" >10.1016/j.funbio.2025.101550</a>
Alternative languages
Result language
angličtina
Original language name
Functional analysis of two genes coding for distinct cation diffusion facilitators of the cadmium-accumulating fungus Agaricus crocodilinus
Original language description
The gilled mushroom Agaricus crocodilinus (Agaricaceae) analyzed in this study hyperaccumulated Cd and showed common Zn and very low Mn concentrations. To gain an insight into the handling of heavy metals in this saprotrophic species, its two genes of the cation diffusion facilitator (CDF) protein family were isolated, AciCDF1 and AciDF2, encoding the membrane transporters of the Zn-CDF and Mn-CDF subfamilies, respectively. When expressed in the model, metal-sensitive yeast, AcCDF1 conferred marked Zn tolerance and promoted the intracellular accumulation of Zn. Green fluorescent protein (GFP) tagging of AcCDF1 visualized the functional protein predominantly in the tonoplast, indicating that AcCDF1 can mediate the transport of Zn into vacuoles, which are used for deposition of excess Zn in most fungi. AcCDF2 conferred a high degree of Mn tolerance to model yeast, in which the transport-active AcCDF2:GFP fusion was localized to the plasma membrane, suggesting a role in Mn export and thus reduced Mn accumulation. Furthermore, the AcCDF2 gene appeared to be Mn-inducible in A. crocodilinus, suggesting an Mn efflux function of AcCDF2. Neither AcCDFs nor the mutant AcCDF1 variants constructed to mimic transmembrane tetrahedral Cd transport sites manifested appreciable Cd-related phenotypes in yeast models, and further efforts are needed to elucidate the mechanism underlying Cd hyperaccumulation in A. crocodilinus.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10511 - Environmental sciences (social aspects to be 5.7)
Result continuities
Project
<a href="/en/project/GA19-06759S" target="_blank" >GA19-06759S: Cadmium hyperaccumulation in macrofungi: from isotopes to proteins and bacterial communities</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Fungal Biology
ISSN
1878-6146
e-ISSN
1878-6162
Volume of the periodical
129
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
101550
UT code for WoS article
001428437300001
EID of the result in the Scopus database
2-s2.0-85217981252