Advantages of the Pre-Immobilization of Enzymes on Porous Supports for Their Entrapment in Sol-Gels

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F05%3A00028621" target="_blank" >RIV/67985858:_____/05:00028621 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Advantages of the Pre-Immobilization of Enzymes on Porous Supports for Their Entrapment in Sol-Gels

Original language description

In this work, we have compared the entrapment of free or immobilized on a porous support (Sepabeads using glutaraldehyde technique) glucose oxidase using a sol-gel technique. The previous immobilization of the enzyme permitted to keep the enzyme activityafter sol-gel formation (only 10% of the enzyme activity was lost whereas the soluble enzyme lost 60% of its initial activity) and to avoid enzyme leakage from the sol-gel matrix, which was relatively high when entrapping the soluble enzyme (39 % of theenzyme activity was released after 16 hr of incubation in a buffered solution). Moreover, the immobilized enzyme, inside the porous support, cannot be in contact with the sol-gel and maintained the stability achieved by multipoint covalent attachment onthe Sepabeads, giving much better stability than the one achieved by trapping the soluble enzyme.

Czech name

Výhody předimobilizace enyzmů na porézním nosiči pro jejich zachycení v sol-gelu

Czech description

V této práci bylo porovnáváno zachycení glucosa oxidasy volné a předimobilizované na Sepabeads do křemičitého gelu. V případě imobilizace volného enzymu aktivita byla sníženo o 60% a docházelo ke ztrátám enzymu únikem z nosiče (39% během 16 hodin). Aktivity přeimobilizovaného enzymu byla snížena pouye o 10%. Mnohabodové kovalentní vázání enyzmu na Sepabeads zvyšuje stabilitu enzymu a zybraňuje jeho úniku.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomacromolecules

ISSN

1525-7797

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

4

Pages from-to

1027-1030

UT code for WoS article

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EID of the result in the Scopus database

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