The strength of integrin binding between neutrophils and endothelial cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985874%3A_____%2F03%3A00375893" target="_blank" >RIV/67985874:_____/03:00375893 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00249-003-0329-4" target="_blank" >http://dx.doi.org/10.1007/s00249-003-0329-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00249-003-0329-4" target="_blank" >10.1007/s00249-003-0329-4</a>
Alternative languages
Result language
angličtina
Original language name
The strength of integrin binding between neutrophils and endothelial cells
Original language description
The firm adhesion of activated polymorphonuclear neutrophils to endothelial cells in blood vessels is achieved through binding of the integrin intercellular adhesion molecule. To contribute to the better understanding of this adhesion step, our investigation is aimed at the relationship between integrin expression and the strength of neutrophil binding to endothelial cells. Flow cytometry and 3D scanning microscopy are used to study integrin expression and distribution, respectively. It is found that CD11b/CD18 integrin expression is localized in clusters distributed irregularly over the neutrophil surface. After cell activation, the cluster distribution polarizes, increasing the local CD11b/CD18 density concurrently with nearly doubled integrin expression. The neutrophil adhesion efficiency is measured in a flow chamber coated successively by various substrates, including endothelial cells in an activated state. Analysis of the flow dependence of the number of attached cells reveals t
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA305%2F01%2F1605" target="_blank" >GA305/01/1605: The effect of revealed receptor clusters on the regulation of adhesiveness and transmigration of white blood cells</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Biophysics Journal With Biophysics Letters
ISSN
0175-7571
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
8
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
684-688
UT code for WoS article
000187309600004
EID of the result in the Scopus database
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