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Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F09%3A00326027" target="_blank" >RIV/67985882:_____/09:00326027 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera

  • Original language description

    In this work, an ptimized SPR assay was implemented and its performance compared with an ELISA assay for CD166/activated cell leukocyte adhesion molecule (ALCAM), as candidate pancreatic cancer marker, based on direct and amplified detection in buffer and in human serum samples from healthy individuals and subjects with cancer. It is demonstrated that SPR can distinguish between ALCAM levels in cancer and control sera using direct detection without the need for additional amplification steps.

  • Czech name

    Srovnávací studie metod SPR a ELISA založená na analýze vzorků krevního séra pacientů s rakovinou a zdravých jedinců na přítomnost CD166/ALCAM

  • Czech description

    V této práci byla metoda SPR porovnána s metodou ELISA pro přímou a amplifikovanou detekci perspektivního biomárkru rakoviny ? CD166 / ALCAM v pufru a ve zředěném lidském krevním séru pacientů s rakovinou a zdravými jedinci. Je ukázáno mmo jiné, že metoda SPR může rozlišit hladiny ALCAMu mezí pozitivními a negativními vzorky séra přímou detekcí bez nutnosti amplifikace.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biosensors and Bioeletronics

  • ISSN

    0956-5663

  • e-ISSN

  • Volume of the periodical

    24

  • Issue of the periodical within the volume

    7

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    6

  • Pages from-to

  • UT code for WoS article

  • EID of the result in the Scopus database