Surface plasmon resonance imaging for parallelized detection of protein biomarkers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F09%3A00345547" target="_blank" >RIV/67985882:_____/09:00345547 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1117/12.820882" target="_blank" >http://dx.doi.org/10.1117/12.820882</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.820882" target="_blank" >10.1117/12.820882</a>

Result language
angličtina
Original language name
Surface plasmon resonance imaging for parallelized detection of protein biomarkers
Original language description
We report a novel high-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of protein biomarkers. The biosensor is based on a high-performance SPR imaging sensor with polarization contrast and internal referencing which yields a considerably higher sensitivity and resolution than conventional SPR imaging systems (refractive index resolution 2 10-7 RIU). We combined the SPR imaging biosensor with microspotting to create an array of antibodies. DNA-directed protein immobilization was utilized for the spatially resolved attachment of antibodies. Using Human Chorionic Gonadotropin (hCG) as model protein biomarker, we demonstrated the potential for simultaneous detection of proteins in up to 100 channels.
Czech name
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Czech description
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Project
<a href="/en/project/KAN200670701" target="_blank" >KAN200670701: Surface plasmon resonance biosensors and protein arrays for medical diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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