Electrochemical surface plasmon resonance biosensor for study of DNA desorption and hybridization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F13%3A00396553" target="_blank" >RIV/67985882:_____/13:00396553 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1117/12.2018462" target="_blank" >http://dx.doi.org/10.1117/12.2018462</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2018462" target="_blank" >10.1117/12.2018462</a>

Result language

angličtina

Original language name

Electrochemical surface plasmon resonance biosensor for study of DNA desorption and hybridization

Original language description

We report a system, which combines electrochemical and surface plasmon resonance (SPR) techniques on the same sensing chip. Each channel of a four-channel laboratory SPR sensor is supplemented with two planar gold electrodes (the reference and the counter electrodes), whereas the gold layer of SPR chip is used as the working electrode. A custom electronics enables to set an arbitrary potential between the reference and working electrodes and to measure the current flow between the counter and the working electrodes. Information from standard electrochemical techniques, i.e. cyclovoltammetry and chronoamperometry can be acquired with the system while simultaneously monitoring the shift in the surface plasmon resonance. The electrochemical SPR biosensorwas used to study desorption of thiolated DNA probes with a negative potential. By comparing the acquired electrochemical and SPR signals, we show that DNA probes as well as a monolayer of alkanethiols can be desorbed by applying negative

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

JA - Electronics and optoelectronics

OECD FORD branch

—

Project

<a href="/en/project/LH11102" target="_blank" >LH11102: Surface Plasmon Resonance Based Biosensing Using Hybrid Nanoplasmonic Materials Fabricated by Self-Assembly Processes</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

OPTICAL SENSORS 2013

ISBN

978-0-8194-9576-1

ISSN

0277-786X

e-ISSN

—

Number of pages

9

Pages from-to

—

Publisher name

SPIE

Place of publication

BELLINGHAM

Event location

Praha

Event date

Apr 15, 2013

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000323326200012