Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F17%3A00484849" target="_blank" >RIV/67985882:_____/17:00484849 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/s41598-017-01229-x" target="_blank" >http://dx.doi.org/10.1038/s41598-017-01229-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-017-01229-x" target="_blank" >10.1038/s41598-017-01229-x</a>

Result language

angličtina

Original language name

Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism

Original language description

In recent years, excessive oxidative metabolism has been reported as a critical determinant of pathogenicity in many diseases. The advent of a simple tool that can provide a physiological readout of oxidative stress would be a major step towards monitoring this dynamic process in biological systems, while also improving our understanding of this process. Ultra-weak photon emission (UPE) has been proposed as a potential tool for measuring oxidative processes due to the association between UPE and reactive oxygen species. Here, we used HL-60 cells as an in vitro model to test the potential of using UPE as readout for dynamically monitoring oxidative stress after inducing respiratory burst. In addition, to probe for possible changes in oxidative metabolism, we performed targeted metabolomics on cell extracts and culture medium. Lastly, we tested the effects of treating cells with the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI). Our results show that UPE can be used as readout for measuring oxidative stress metabolism and related processes

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10306 - Optics (including laser optics and quantum optics)

Project

<a href="/en/project/GA13-29294S" target="_blank" >GA13-29294S: Photonic biosignals: measurement and characterization</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

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UT code for WoS article

000400189700006

EID of the result in the Scopus database

2-s2.0-85018387430