Interaction of Tris with DNA molecules and carboxylic groups on self-assembled monolayers of alkanethiols measured with surface plasmon resonance

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F21%3A00543285" target="_blank" >RIV/67985882:_____/21:00543285 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.apsusc.2021.148984" target="_blank" >https://doi.org/10.1016/j.apsusc.2021.148984</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.apsusc.2021.148984" target="_blank" >10.1016/j.apsusc.2021.148984</a>

Result language
angličtina
Original language name
Interaction of Tris with DNA molecules and carboxylic groups on self-assembled monolayers of alkanethiols measured with surface plasmon resonance
Original language description
Functional materials employing organic coatings on inorganic substrates are perceived as potential platforms for applications in a variety of fields. Therefore, the investigation of interactions of such systems with the micro-environment has become an important research direction in surface science. Herein, we study the interaction of one of the buffers most commonly used in biological studies, Tris buffer, with self-assembled monolayers (SAMs) of alkanethiols and short DNAs using a surface plasmon resonance (SPR) biosensor. We show that the interaction between Tris and carboxylic groups of SAMs is a complex multiphasic process. We demonstrate that Tris base binds to the protonated carboxylic groups. When those groups become deprotonated, Tris base dissociates and Tris acid is attracted, which results in the formation of a diffuse layer over the charged surface. In addition, we show that the interaction of Tris with the immobilized DNA molecules biases the determination of surface concentrations of the immobilized DNA molecules and thus also the determination of hybridization efficiencies
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Project
<a href="/en/project/GA19-02739S" target="_blank" >GA19-02739S: Advanced plasmonic biosensors: towards the next-generation biomolecular interaction analysis</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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