Lab-on-chip microscope platform for electro-manipulation of a dense microtubules network
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F22%3A00567065" target="_blank" >RIV/67985882:_____/22:00567065 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/22:00564452 RIV/67985823:_____/22:00564452
Result on the web
<a href="https://doi.org/10.1038/s41598-022-06255-y" target="_blank" >https://doi.org/10.1038/s41598-022-06255-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-022-06255-y" target="_blank" >10.1038/s41598-022-06255-y</a>
Alternative languages
Result language
angličtina
Original language name
Lab-on-chip microscope platform for electro-manipulation of a dense microtubules network
Original language description
Pulsed electric field (PEF) technology is promising for the manipulation of biomolecular components and has potential applications in biomedicine and bionanotechnology. Microtubules, nanoscopic tubular structures self-assembled from protein tubulin, serve as important components in basic cellular processes as well as in engineered biomolecular nanosystems. Recent studies in cell-based models have demonstrated that PEF affects the cytoskeleton, including microtubules. However, the direct effects of PEF on microtubules are not clear. In this work, we developed a lab-on-a-chip platform integrated with a total internal reflection fluorescence microscope system to elucidate the PEF effects on a microtubules network mimicking the cell-like density of microtubules. The designed platform enables the delivery of short (microsecond-scale), high-field-strength (<= 25 kV/cm) electric pulses far from the electrode/electrolyte interface. We showed that microsecond PEF is capable of overcoming the non-covalent microtubule bonding force to the substrate and translocating the microtubules. This microsecond PEF effect combined with macromolecular crowding led to aggregation of microtubules. Our results expand the toolbox of bioelectronics technologies and electromagnetic tools for the manipulation of biomolecular nanoscopic systems and contribute to the understanding of microsecond PEF effects on a microtubule cytoskeleton
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
20201 - Electrical and electronic engineering
Result continuities
Project
<a href="/en/project/GA18-23597S" target="_blank" >GA18-23597S: High-frequency microdevices for controlling protein nanomotors</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
12
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
2462
UT code for WoS article
000755212600015
EID of the result in the Scopus database
2-s2.0-85124615232