Biological autoluminescence enables effective monitoring of yeast cell electroporation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F24%3A00585597" target="_blank" >RIV/67985882:_____/24:00585597 - isvavai.cz</a>
Result on the web
<a href="https://analyticalsciencejournals-onlinelibrary-wiley-com.ezproxy.techlib.cz/doi/10.1002/biot.202300475" target="_blank" >https://analyticalsciencejournals-onlinelibrary-wiley-com.ezproxy.techlib.cz/doi/10.1002/biot.202300475</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/biot.202300475" target="_blank" >10.1002/biot.202300475</a>
Alternative languages
Result language
angličtina
Original language name
Biological autoluminescence enables effective monitoring of yeast cell electroporation
Original language description
The application of pulsed electric fields (PEFs) is becoming a promising tool for application in biotechnology, and the food industry. However, real-time monitoring of the efficiency of PEF treatment conditions is challenging, especially at the industrial scale and in continuous production conditions. To overcome this challenge, we have developed a straightforward setup capable of real-time detection of yeast biological autoluminescence (BAL) during pulsing. Saccharomyces cerevisiae culture was exposed to 8 pulses of 100 mu s width with electric field strength magnitude 2-7 kV cm-1. To assess the sensitivity of our method in detecting yeast electroporation, we conducted a comparison with established methods including impedance measurements, propidium iodide uptake, cell growth assay, and fluorescence microscopy. Our results demonstrate that yeast electroporation can be instantaneously monitored during pulsing, making it highly suitable for industrial applications. Furthermore, the simplicity of our setup facilitates its integration into continuous liquid flow systems. Additionally, we have established quantitative indicators based on a thorough statistical analysis of the data that can be implemented through a dedicated machine interface, providing efficiency indicators for analysis.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/GX20-06873X" target="_blank" >GX20-06873X: SubTHz on-chip devices for controlling protein nanomachines</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biotechnology Journal
ISSN
1860-6768
e-ISSN
1860-7314
Volume of the periodical
19
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
e2300475
UT code for WoS article
001206049200001
EID of the result in the Scopus database
2-s2.0-85191046037