Positive impact of dynamic seeding of mesenchymal stem cells on bone-like biodegradable scaffolds with increased content of calcium phosphate nanoparticles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F19%3A00511599" target="_blank" >RIV/67985891:_____/19:00511599 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/19:00511599 RIV/68407700:21220/19:00334696 RIV/00216208:11110/19:10396068 RIV/00216208:11140/19:10396068 RIV/00064165:_____/19:10396068

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs11033-019-04903-7" target="_blank" >https://link.springer.com/article/10.1007%2Fs11033-019-04903-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11033-019-04903-7" target="_blank" >10.1007/s11033-019-04903-7</a>

Result language

angličtina

Original language name

Positive impact of dynamic seeding of mesenchymal stem cells on bone-like biodegradable scaffolds with increased content of calcium phosphate nanoparticles

Original language description

One of the main aims of bone tissue engineering, regenerative medicine and cell therapy is development of an optimal artificial environment (scaffold) that can trigger a favorable response within the host tissue, it is well colonized by resident cells of organism and ideally, it can be in vitro pre-colonized by cells of interest to intensify the process of tissue regeneration. The aim of this study was to develop an effective tool for regenerative medicine, which combines the optimal bone-like scaffold and colonization technique suitable for cell application. Accordingly, this study includes material (physical, chemical and structural) and in vitro biological evaluation of scaffolds prior to in vivo study. Thus, porosity, permeability or elasticity of two types of bone-like scaffolds differing in the ratio of collagen type I and natural calcium phosphate nanoparticles (bCaP) were determined, then analyzes of scaffold interaction with mesenchymal stem cells (MSCs) were performed. Simultaneously, dynamic seeding using a perfusion bioreactor followed by static cultivation was compared with standard static cultivation for the whole period of cultivation. In summary, cell colonization ability was estimated by determination of cell distribution within the scaffold (number, depth and homogeneity), matrix metalloproteinase activity and gene expression analysis of signaling molecules and differentiation markers. Results showed, the used dynamic colonization technique together with the newly-developed collagen-based scaffold with high content of bCaP to be an effective combined tool for producing bone grafts for bone implantology and regenerative medicine.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20601 - Medical engineering

Project

<a href="/en/project/NV15-25813A" target="_blank" >NV15-25813A: Development and comprehensive pre-clinical testing of novel composite materials for bone surgery</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Biology Reports

ISSN

0301-4851

e-ISSN

—

Volume of the periodical

46

Issue of the periodical within the volume

4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

18

Pages from-to

4483-4500

UT code for WoS article

000478684300081

EID of the result in the Scopus database

2-s2.0-85067071156