Peptide mass mapping in bioapatites isolated from animal bones

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F20%3A00531743" target="_blank" >RIV/67985891:_____/20:00531743 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/20:43921498

Result on the web

<a href="https://link.springer.com/article/10.1007/s10856-020-06371-z" target="_blank" >https://link.springer.com/article/10.1007/s10856-020-06371-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10856-020-06371-z" target="_blank" >10.1007/s10856-020-06371-z</a>

Result language

angličtina

Original language name

Peptide mass mapping in bioapatites isolated from animal bones

Original language description

Bioapatite ceramics produced from biogenic sources provide highly attractive materials for the preparation of artificial replacements since such materials are not only more easily accepted by living organisms, but bioapatite isolated from biowaste such as xenogeneous bones also provides a low-cost material. Nevertheless, the presence of organic compounds in the bioapatite may lead to a deterioration in its quality and may trigger an undesirable immune response. Therefore, procedures which ensure the elimination of organic compounds through bioapatite isolation are being subjected to intense investigation and the presence of remaining organic impurities is being determined through the application of various methods. Since current conclusions concerning the conditions suitable for the elimination of organic compounds remain ambiguous, we used the mass spectrometry-based proteomic approach in order to determine the presence of proteins or peptides in bioapatite samples treated under the most frequently employed conditions, i.e., the alkaline hydrothermal proces and calcination at 500 °C. Since we also investigated the presence of proteins or peptides in treated bioapatite particles of differing sizes, we discovered that both calcination and the size of the bioapatite particles constitute the main factors influencing the presence of proteins or peptides in bioapatite. In fact, while intact proteins were detected even in calcinated bioapatite consisting of particles >250 μm, no proteins were detected in the same material consisting of particles <40 μm. Therefore, we recommend the use of powdered bioapatite for the preparation of artificial replacements since it is, more effectively purified than apatite in the form of blocks. In addition, we observed that while alkaline hydrothermal treatment leads to the non-specific cleavage of proteins, it does not ensure the full degradation thereof.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20601 - Medical engineering

Project

<a href="/en/project/NV15-25813A" target="_blank" >NV15-25813A: Development and comprehensive pre-clinical testing of novel composite materials for bone surgery</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Materials Science-Materials in Medicine

ISSN

0957-4530

e-ISSN

—

Volume of the periodical

31

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

32

UT code for WoS article

000521322700001

EID of the result in the Scopus database

2-s2.0-85081173067