A New Method for the Production of High-Concentration Collagen Bioinks with Semiautonomic Preparation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F24%3A00584953" target="_blank" >RIV/67985891:_____/24:00584953 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/24:00371291
Result on the web
<a href="https://doi.org/10.3390/gels10010066" target="_blank" >https://doi.org/10.3390/gels10010066</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/gels10010066" target="_blank" >10.3390/gels10010066</a>
Alternative languages
Result language
angličtina
Original language name
A New Method for the Production of High-Concentration Collagen Bioinks with Semiautonomic Preparation
Original language description
It is believed that 3D bioprinting will greatly help the field of tissue engineering and regenerative medicine, as live patient cells are incorporated into the material, which directly creates a 3D structure. Thus, this method has potential in many types of human body tissues. Collagen provides an advantage, as it is the most common extracellular matrix present in all kinds of tissues and is, therefore, very natural for cells and the organism. Hydrogels with highly concentrated collagen make it possible to create 3D structures without additional additives to crosslink the polymer, which could negatively affect cell proliferation and viability. This study established a new method for preparing highly concentrated collagen bioinks, which does not negatively affect cell proliferation and viability. The method is based on two successive neutralizations of the prepared hydrogel using the bicarbonate buffering mechanisms of the 2× enhanced culture medium and pH adjustment by adding NaOH. Collagen hydrogel was used in concentrations of 20 and 30 mg/mL dissolved in acetic acid with a concentration of 0.05 and 0.1 wt.%. The bioink preparation process is automated, including colorimetric pH detection and adjustment. The new method was validated using bioprinting and subsequent cultivation of collagen hydrogels with incorporated stromal cells. After 96 h of cultivation, cell proliferation and viability were not statistically significantly reduced.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/NV19-02-00068" target="_blank" >NV19-02-00068: Bioartificial cardiovascular patches and vessels from porcine collagen reinforced with nano/microfibers using stem cells and dynamic culture</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Gels
ISSN
2310-2861
e-ISSN
2310-2861
Volume of the periodical
10
Issue of the periodical within the volume
1
Country of publishing house
CH - SWITZERLAND
Number of pages
23
Pages from-to
66
UT code for WoS article
001149227800001
EID of the result in the Scopus database
2-s2.0-85183415558