The comparison of eight different common in vitro and ex vivo environmentsnwith in vivo conditions applying model collagen samples: Correlationnpossibilities and their limits

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F24%3A00600196" target="_blank" >RIV/67985891:_____/24:00600196 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.polymertesting.2024.108621" target="_blank" >https://doi.org/10.1016/j.polymertesting.2024.108621</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.polymertesting.2024.108621" target="_blank" >10.1016/j.polymertesting.2024.108621</a>

Result language

angličtina

Original language name

The comparison of eight different common in vitro and ex vivo environmentsnwith in vivo conditions applying model collagen samples: Correlationnpossibilities and their limits

Original language description

New biomaterials are routinely evaluated for their degradation behaviour in the real body environment. Following the 3R strategy, in vitro simulated body conditions are often preferred. No studies that simultaneously compare such conditions with the real body environment have been conducted to date. Model porous collagen scaffolds were exposed for 21 days to eight different environments: simple salt-based and enzymatic media, human blood plasma, cell culture media with and without human fibroblasts and ex vivo model cortical bone, and subsequently compared with an in vivo environment represented by a pig peritoneum. The mechanical properties of the scaffolds were then determined via uniaxial compression testing, and the structural properties via the micro-CT, weight loss, infrared spectroscopy, X-ray diffraction and histological methods. Interestingly, the various analysed simulated body conditions caused differing alterations in the collagen scaffold characteristics when compared with the real body environment. The mechanical properties were similar during the first 7 days of incubation but diverged after 14 and 21 days. The structural properties varied significantly after just 7 days of incubation. The histological evaluation of the scaffolds exposed to the cellular, ex vivo and in vivo conditions revealed the poor ability of cells to completely populate the scaffolds, accompanied by the massive ingrowth of connective tissue into the in vivonexposed scaffolds, which resulted in their variable global behaviour. In conclusion, the value of in vitro simulated body environments lies in their screening capacity and feasibility, however, direct extrapolation to real body conditions needs to be verified going forward.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20601 - Medical engineering

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Polymer Testing

ISSN

0142-9418

e-ISSN

1873-2348

Volume of the periodical

140

Issue of the periodical within the volume

November

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

17

Pages from-to

108621

UT code for WoS article

001348383400001

EID of the result in the Scopus database

2-s2.0-85207337208