Relocalization of nuclear DNA helicase II during the growth period of bovine oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F06%3A00040778" target="_blank" >RIV/67985904:_____/06:00040778 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Relocalization of nuclear DNA helicase II during the growth period of bovine oocytes

Original language description

Nuclear DNA helicase II (NDH II) is the bovine homolog of human RNA helicase A. The aim of this study was to compare NDH II localization between somatic cells (bovine embryonal fibroblasts) and female germ cells (oocytes), with the main focus on the dynamic changes in the redistribution of NDH II during the growth phase of the bovine oocytes. The fine granular staining of NDH II was spread in the whole nucleoplasm of fibroblasts, excluding the reticulated nucleoli. In contrast, the large reticulated nucleoli of the growing oocytes isolated from early antral follicles exhibited strong positivity for NDH II together with the immunostaining signals of upstream binding factor (UBF) and RNA polymerase I subunit (PAF53), documenting the high synthetic activity of these nucleoli. At the time of termination of oocyte growth, NDH II was preferentially located at the nucleolar periphery together with proteins of fibrillar centres. In fully grown oocytes, NDH II was still present in the thin peri

Czech name

Změny lokalizace jaderné DNA helikasy II v průběhu růstové periody bovinních oocytů

Czech description

Cílem práce bylo srovnat lokalizaci nukleární DNA helikasyII u somatických buněk ( bovinní embryonální fibroblasty) a samičích zárodečných buněk ( oocytů) s hlavním důrazem na dynamické změny v redistribuci v průběhu růstové fáze bovinních oocytů. V časeukončení růstu oocytů , oproti předchozí fázi, byla NDHII přednostně lokalizována na periferii jadérka spolu s proteiny fibrilárních center.Tyto výsledky spolu s kvantitativními údaji NDHII exprese na úrovni mRNA a proteinu demonstrují ,že exprese NDHIIje paralelní k aktivite ribosomální RNA biosyntezy.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA523%2F03%2F0857" target="_blank" >GA523/03/0857: The role of Akt kinase in the regulation of G2/M cell cycle transition and in the acquisition of developmental potency of oocytes</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Histochemistry and Cell Biology

ISSN

0948-6143

e-ISSN

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Volume of the periodical

125

Issue of the periodical within the volume

1-2

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

155-164

UT code for WoS article

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EID of the result in the Scopus database

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