Regulation of cap-dependent translation initiation in the early stage porcine parthenotes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F08%3A00312613" target="_blank" >RIV/67985904:_____/08:00312613 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Regulation of cap-dependent translation initiation in the early stage porcine parthenotes
Original language description
In this study we document that both the translation initiation factor eIF4E and its regulator 4E-BP1 become dephosphorylated in the early stage porcine zygotes already 8 hr post-activation.Similarly, the activities of ERK1/2 MAP and Mnk1 kinases, which are both involved in eIF4E phosphorylation, gradually decrease during this period with the timing similar to that of eIF4E dephosphorylation. The formation of an active eIF4F complex is also diminished after 9-15 hr post-activation, although substantial amounts of this complex have been detected also 24 hr post-activation (2-cell stage). The overall protein synthesis in the parthenotes decreases gradually from 12 hr post-activation reaching a minimum after 48 hr (4-cell stage). Although the translation is gradually decreasing during early preimplantation development, the eIF4F complex, which is temporarily formed, might be a premise for the translation of a small subset of mRNAs at this period of development.
Czech name
Regulace na čepičce závislé translační iniciace závislé v časném prasečím partenotu
Czech description
V této studii dokumentujeme, že translační iniciační faktor eIF4E a jeho regulátor 4E-BP1 jsou defosforylovány v časných prasečích zygotách již za 8 hod po aktivaci. Současně se snižuje aktivita ERK1/2 MAP and Mnk1 kináz. Tvorba aktivního eIF4F komplexuje rovněž snížena 9-15 hod po aktivaci ale za 24 hod je opět detekována jeho podstatná hladina. Proteosyntéza průběžně klesá do 48 hod.. Přestože je translace v preimplantačním stadiu průběžně snižována, může mít přechodná tvorba eIF4F komplexu vliv na translaci malého subsetu mRNA v této vývojové periodě.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Reproduction and Development
ISSN
1040-452X
e-ISSN
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Volume of the periodical
75
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
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UT code for WoS article
000260873200004
EID of the result in the Scopus database
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