Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F11%3A00359775" target="_blank" >RIV/67985904:_____/11:00359775 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1530/REP-10-0418" target="_blank" >http://dx.doi.org/10.1530/REP-10-0418</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1530/REP-10-0418" target="_blank" >10.1530/REP-10-0418</a>

Result language

angličtina

Original language name

Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins

Original language description

The aim of this work was to assess the FSH-stimulated expression of epidermal growth factor (EGF)-like peptides in cultured cumulus-oocyte complexes (COCs) and to find out the effect of the peptides on cumulus expansion, oocyte maturation and acquisitionof developmental competence in vitro. FSH promptly stimulated expression of amphiregulin (AREG) and epiregulin (EREG), but notbetacellulin (BTC) in the cultured COCs. Both FSH and the EGF-like peptides significantly stimulated expression of expansion related genes (PTGS2, TNFAIP6 and HAS2) in the COCs and stimulated maturation of oocytes and expansion of cumulus cells. In contrast to FSH, AREG and EREG neither stimulated expression of CYP11A1 in the COCs nor an increase in progesterone production by cumulus cells. Significantly more oocytes stimulated with AREG and/or EREG developed to blastocyst stage after parthenogenetic activation when compared to oocytes stimulated with FSH alone or combinations of FSH/LH or PMSG/hCG.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Reproduction

ISSN

1470-1626

e-ISSN

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Volume of the periodical

141

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

425-435

UT code for WoS article

000288500500005

EID of the result in the Scopus database

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