Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F12%3A00378006" target="_blank" >RIV/67985904:_____/12:00378006 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1073/pnas.1117018109" target="_blank" >http://dx.doi.org/10.1073/pnas.1117018109</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.1117018109" target="_blank" >10.1073/pnas.1117018109</a>
Alternative languages
Result language
angličtina
Original language name
Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi
Original language description
Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). Although the protein-coding gene regions often had a higher percent of correct identification comparedwith ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest p
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
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Volume of the periodical
109
Issue of the periodical within the volume
16
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
6241-6246
UT code for WoS article
000303246100068
EID of the result in the Scopus database
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