PCR detection of uncultured rumen bacteria

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F12%3A00379145" target="_blank" >RIV/67985904:_____/12:00379145 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s12223-012-0135-4" target="_blank" >http://dx.doi.org/10.1007/s12223-012-0135-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12223-012-0135-4" target="_blank" >10.1007/s12223-012-0135-4</a>

Result language
angličtina
Original language name
PCR detection of uncultured rumen bacteria
Original language description
16S rRNA sequences of ruminal uncultured bacterial clones from public databases were phylogenetically examined. The sequences were found to form two unique clusters not affiliated with any known bacterial species: cluster of unidentified sequences of free floating rumen fluid uncultured bacteria (FUB) and cluster of unidentified sequences of bacteria associated with rumen epithelium (AUB). A set of PCR primers targeting 16S rRNA of ruminal free uncultured bacteria and rumen epithelium adhering uncultured bacteria was designed based on these sequences. FUB primers were used for relative quantification of uncultured bacteria in ovine rumen samples. The effort to increase the population size of FUB group has been successful in sulfate reducing broth and culture media supplied with cellulose.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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