Aurora kinase a drives mtoc biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F12%3A00384274" target="_blank" >RIV/67985904:_____/12:00384274 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1095/biolreprod.112.101014" target="_blank" >http://dx.doi.org/10.1095/biolreprod.112.101014</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1095/biolreprod.112.101014" target="_blank" >10.1095/biolreprod.112.101014</a>

Result language

angličtina

Original language name

Aurora kinase a drives mtoc biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo

Original language description

Aurora kinase A (AURKA) is an important mitotic kinase involved in the G2/M transition, centrosome maturation and separation, and spindle formation in somatic cells. We used transgenic models that specifically overexpress in mouse oocytes either wild-type (WT-AURKA) or a catalytically inactive (kinasedead) (KD-AURKA) AURKA to gain new insights regarding the role of AURKA during oocyte maturation. AURKA activation occurs shortly after hCG administration that initiates maturation in vivo. Although AURKA activity is increased in WT-AURKA oocytes, resumption of meiosis is not observed in the absence of hCG administration. Control oocytes contain one to three microtubule organizing centers (MTOCs; centrosome equivalent) at prophase I. At the time of germinal vesicle breakdown (GVBD), the first visible marker of resumption of meiosis, the MTOC number increases. In WT-AURKA oocytes, the increase in MTOC number occurs prematurely but transiently without GVBD, whereas the increase in MTOC numbe

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biology of Reproduction

ISSN

0006-3363

e-ISSN

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Volume of the periodical

87

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

1-12

UT code for WoS article

000314231000009

EID of the result in the Scopus database

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