Polo-like kinase 1 is essential for the first mitotic division in the mouse embryo

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F13%3A00396534" target="_blank" >RIV/67985904:_____/13:00396534 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/mrd.22188" target="_blank" >http://dx.doi.org/10.1002/mrd.22188</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mrd.22188" target="_blank" >10.1002/mrd.22188</a>

Result language

angličtina

Original language name

Polo-like kinase 1 is essential for the first mitotic division in the mouse embryo

Original language description

Polo-like kinase 1 (PLK1), a member of the serine/threonine protein kinases family, is involved in multiple steps of mitotic progression. It regulates centrosome maturation, mitotic spindle formation, and cytokinesis. While studied extensively in somaticcells, little is known about PLK1 activities in the mammalian preimplantation embryo. We examined the role of PLK1 in the one-cell mouse embryo. Western blotting showed that the PLK1 protein content increased significantly during the S-phase of the one-cell stage and declined during the first mitotic division. Activation of PLK1 preceded nuclear envelope breakdown (NEBD) in both pronuclei at the entry to first embryo mitosis. Immunofluorescence revealed the presence of phosphorylated, active PLK1 (pThr(210) -PLK1) in both male and female pronuclei, and in the microtubule-organizing centers (MTOCs) shortly before NEBD. During the first mitotic metaphase, pThr(210) -PLK1 accumulated at the spindle poles and was also associated with conde

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Reproduction and Development

ISSN

1040-452X

e-ISSN

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Volume of the periodical

80

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

522-534

UT code for WoS article

000328127800003

EID of the result in the Scopus database

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